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-tag certain immunoblot Evaluation of RAW264.7 cell lysate the αT2ib protein band was visualized by application of a mouse anti-

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Developing an adenoviral vector carrying a bicystronic expression cassette driving expression of ER intrabody αT2ib along with the reporter gene EGFP a strategy comprising two cloning techniques was pursued. In the initial step the intrabody expression cassette was cloned in to the vector pGEM/IRES/EGFP [43] that contains the IRES sequence in the poliovirus accompanied by the reporter gene EGFP.

The generated anti-TLR2 scFv intrabody inhibits exclusively and very successfully TLR2 ligand-driven mobile activation in vitro and ex vivo

To translate benefits of our analyses in vitro for the systemic amount, we infected naive mice with AdVαT2ib by i. v. injection, in addition to mice that were thioglycolate challenged 3 times previously by i. p. injection of your adenovirus. six d following an infection EGFP expression as a sign of αT2ib expression was evident on infection i.v. in splenocytes irrespective of whether mice had been contaminated with AdVαT2ib or AdVGFP.

Subcellular colocalisation and co-immunoprecipitation of αT2ib and mouse/human TLR2 on transfection. A

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